Multimodality Rodent Imaging Chambers for Use Under Barrier Conditions with Gas Anesthesia

Purpose  The ability to reproducibly and repeatedly image rodents in noninvasive imaging systems, such as small-animal positron emission tomography (PET) and computed tomography (CT), requires a reliable method for anesthetizing, positioning, and heating animals in a simple reproducible manner. In this paper, we demonstrate that mice and rats can be reproducibly and repeatedly imaged using an imaging chamber designed to be rigidly mounted on multiple imaging systems. Procedures  Mouse and rat imaging chambers were made of acrylic plastic and aluminum. MicroCT scans were used to evaluate the positioning reproducibility of the chambers in multimodality and longitudinal imaging studies. The ability of the chambers to maintain mouse and rat body temperatures while anesthetized with gas anesthesia was also evaluated. Results  Both the mouse and rat imaging chambers were able to reproducibly position the animals in the imaging systems with a small degree of error. Placement of the mouse in the mouse imaging chamber resulted in a mean distance of 0.23 mm per reference point in multimodality studies, whereas for longitudinal studies the mean difference was 1.11 mm. The rat chamber resulted in a mean difference of 0.46 mm in multimodality studies and a mean difference of 4.31 mm in longitudinal studies per reference point. The chambers maintained rodent body temperatures at the set point temperature of 38°C. Conclusions  The rodent imaging chambers were able to reproducibly position rodents in tomographs with a small degree of variability and were compatible with routine use. The embedded anesthetic line and heating system was capable of maintaining the rodent’s temperature and anesthetic state, thereby enhancing rodent health and improving data collection reliability.     

重度妊高征持续性硬膜外麻醉剖宫产血压变化的临床分析

目的 :了解重度妊高征持续性硬膜外麻醉下剖宫产时的血压变化规律。方法 :选择重度妊高征孕妇和正常孕妇各 1 0 2例 ,比较持续性硬膜外麻醉下剖宫产术前、术中最低、麻醉后、术后的血压变化及术中输液量。结果 :麻醉后妊高征组与对照组的最低血压及麻醉后血压均较麻醉前血压显著降低 (P<0 .0 1 ) ;血压下降幅度妊高征组显著大于对照组 (P<0 .0 1 ) ;术中输液量妊高征组显著高于对照组 (P<0 .0 5) ;两组最低血压中舒张压相近 (P>0 .0 5) ;术后血压回升稍高于麻醉前血压。结论 :持续性硬膜外麻醉能够暂时解除血管痉挛及降低血管的紧张度 ,使血压下降 ;术后血管紧张度恢复及术中输液 ,造成术后有效循环血容量相对增加 ,致使血压回升 ,诱发子痫。     

异丙酚及伍用芬太尼或氯胺酮在纤维结肠镜检查麻醉中的临床效果比较

目的：观察和比较异丙酚及伍用芬太尼或氨胺酮在纤维结肠镜检查静脉麻醉中的效果。方法：选择择期纤维结肠镜检查患者60例，随机分为3组，每组20例。P组用异丙酚，F组用异丙酚＋芬太尼，K组用异丙酚＋氯胺酮。记录和比较诱导时间、异丙酚诱导剂量、维持剂量、恢复时间、患者血压和心率变化、临床效果和并发症发生率。结果：F组和K组诱导时间比P组明显缩短（P＜0．01），诱导剂量比P组明显减少（P＜0．01），K组异丙酚诱导剂量和维持剂量比其它两组均明显减少（P＜0．01）；F组和K组睁眼时间、定向能力恢复时间比P组明显延长（P＜0．01）；F组和P组诱导后平均动脉压明显下降（P＜0．01），F组心率也同时下降（P＜0．01），K组血压、心率无明显变化；K组临床麻醉效果比P组和F组好（P＜0．01）；F组呼吸抑制发生率高于其它两组（P＜0．05），各组间其它并发症发生率无明显差异。结论：3组都能提供较好的纤维结肠镜检查条件，而单用异内酚及伍用芬太尼麻醉时呼吸、循环改变明显，异丙酚伍用氯胺酮虽然恢复时间稍有延长，但生命体征稳定，麻醉效果更好。     

混合酸对狗牙—介质—颌骨的石蜡切片制作的影响

目的 探讨混合酸锐钙时间及脱钙温度对切片质量的影响。方法 通过脱钙，记录脱钙时间，温度，制作石蜡切片，HE染色，显微镜观察等步骤，比较混合酸的脱钙时间及脱钙温度对切片质量造成的影响。结果 室温越高，脱钙时间越短，染色效果越好。结论 室温时，最佳脱钙时间为8～9d，选择合适的混合酸，保持其浓度能取得更好的石蜡切片。     

混合性结缔组织病30例临床分析

目的探讨混合性结缔组织病(MCTD)患者临床特点与病程的相关性。方法对明确诊断MCTD的30例住院病例进行了回顾性分析。将其分为两组A组病程＜5年，B组病程≥5年。结果MCTD首发症状主要为雷诺现象、关节痛/关节炎等，其临床表现A组雷诺现象发生率非常显著高于B组(P＜0．01)，而后者肺动脉高压/肺间质纤维化发生率显著增加(P＜0．05)。实验室指标中IgG、ESR异常发生率高，肾损(尿蛋白异常)发生率为26．7％。A组无一例死亡，而B组死亡率为25％。结论MCTD并非一良性疾病，随着病程延长，其重要脏器受损发生率增加且死亡率有增高趋势。     

Anesthesia Management of Living RelatedLiver Transplantation(A case report)

Thelivingrelatedlivertransplantation,anewtechniqueoflivertransplantation,wassuccessfullyperformedinourhospital.Detailsoftheanesthesiamanagementwerereported.PatientsandMaterialsA30yearsoldmalepatientweighting45kgwasscheduledtoreceiveatotalliverresectionandlivingrelatedlefthemilivertransplantation.SeverelivercirrhosisandhepaticcancerwereconfirmedbypreoperativeCTscanningandtwo-dimensionultrasonicexamination.Themasswasabout20cm×22cm×15cmintherightlobe.AFPwasmorethan400μg/L.ALT,AST,GGTwer…     

异丙酚静脉麻醉在颅脑外伤手术中的应用体会

（目的）比较民丙酚与硫喷妥钠在静脉麻醉诱导时意识消失时间、气管插管反应及清醒时间（停止注药到拔管）。（方法）选择Glasgow评分8分以上的颅脑外伤手术患者60例，随机分为两组。A组用异丙酚麻醉，B组用硫喷妥钠麻醉。（结果）A组意识消失时间明显快于B组（P＜0．05），气管插管评级两组比较差异无显著性（P＞0．05）。A组清酷暑时明显快于B组，两组间差异有非常显著性（P＜0．01）。（结论）异丙酚作用快、维持时间短、体内无蓄积、毒性小、恢复迅速平稳，是一种安全有效的静脉麻醉药。     

雷米芬太尼辅助全麻诱导在腹腔镜短小手术中的应用

目的:探讨雷米芬太尼辅助全麻诱导在腹腔镜短小手术中的应用效果.方法:选择腹腔镜短小手术患者2 000例,随机分为雷米芬太尼辅助诱导Ⅰ组和常规诱导Ⅱ组,全麻诱导期每间隔1 min测量1次血压,全麻维持期、苏醒期每间隔5 min测量1次血压,记录HR、MAP、复苏期的呼吸频率、脉搏血氧饱和度.结果:麻醉诱导面罩加压给氧期、...     

Interference in acetylene intersystem crossing acts as the molecular analog of Young's double-slit experiment

We report on an experimental approach that reveals crucial details of the composition of singlet-triplet mixed eigenstates in acetylene. Intersystem crossing in this prototypical polyatomic molecule embodies the mixing of the lowest excited singlet state (S₁) with 3 triplet states (T₁, T₂, and T₃). Using high-energy (157-nm) photons from an F₂ laser to record excited-state photoelectron spectra, we have decomposed the mixed eigenstates into their S₁, T₃, T₂, and T₁ constituent parts. One example of the interpretive power that ensues from the selective sensitivity of the experiment to the individual electronic state characters is the discovery and examination of destructive interference between two doorway-mediated intersystem crossing pathways. This observation of an interference effect in nonradiative decay opens up possibilities for rational coherent control over molecular excited state dynamics.     

针刺复合硬膜外麻醉下小切口胆囊切除术的研究

目的 探讨针刺复合硬膜外麻醉外小切口胆囊切除术是否适宜于基层医院推广应用。方法 选择四所基层医院开展针刺复合硬膜外麻醉下小切口胆囊切除术（试验组300例），其结果与我院同期完成的该手术（对照组280例）结果进行对照分析。结果 试验组中291例完成针刺复合硬膜外麻醉下小切口胆囊切除术，成功率97％，并发症率12％；对照组中276例完成该手术成功率98．4％，并发症率11．4％，两组均未发生出血、胆管损伤和其他严重并发症。结论 经过技术上改进，基层医院开展针刺复合硬膜外麻醉下小切口胆囊切除术是可行的。